American University of Sharjah 1 article published in JoVE Neuroscience Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain Reem Khalil1, Jonathan B. Levitt2 1Department of Biology, Chemistry and Environmental Sciences, American University of Sharjah, 2Department of Biology, The City College of New York We describe a histochemical procedure that reveals characteristic laminar and areal zinc staining patterns in different brain regions. The zinc-staining pattern may be used in conjunction with other anatomical markers to reliably distinguish layers and regions in the developing and adult brain.