Cambridge Institute of Science 1 article published in JoVE Neuroscience The "Brain Milking" Method for the Isolation of Neural Stem Cells and Oligodendrocyte Progenitor Cells from Live Rats Dimitrios Dimitrakopoulos1, Chrisitna Dimitriou1, Freyja McClenahan2, Robin J. M. Franklin2,3, Ilias Kazanis1 1Laboratory of Developmental Biology, Department of Biology, University of Patras, 2Wellcome Trust-MRC Cambridge Stem Cell Institute, University of Cambridge, 3Altos Labs, Cambridge Institute of Science A method for the isolation of neural stem cells and oligodendrocyte progenitor cells from the brains of live rats is presented here in experimental detail. It allows multiple collections of these cells from the same animals without compromising their well-being.
