Appalachian State University 5 articles published in JoVE Biology Profiling the Bacterial Community of Fermenting Traminette Grapes during Wine Production using Metagenomic Amplicon Sequencing Adrienne Goppold1, Louis Conradie1, Otávio Guilherme Gonçalves de Almeida2, Elaine Cristina Pereira De Martinis2, Folarin Anthony Oguntoyinbo1 1A.R. Smith Department of Chemistry and Fermentation Sciences, Appalachian State University, 2Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo Here, we present a protocol to describe amplicon metagenomic for determining the bacterial community of Traminette grapes, fermenting grapes, and final wine. Biology A Simple and Inexpensive Running Wheel Model for Progressive Resistance Training in Mice Pieter J. Koopmans1,2, Kevin A. Zwetsloot1,2,3 1Integrative Muscle Physiology Laboratory, Appalachian State University, 2Department of Health and Exercise Science, Appalachian State University, 3Department of Biology, Appalachian State University This procedure describes a translatable progressive loaded running wheel resistance training model in mice. The primary advantage of this resistance training model is that it is entirely voluntary, thus reducing stress for the animals and the burden on the researcher. Immunology and Infection Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood Jennifer E. McBride1, Mary P. Meaney2, Casey John2, David C. Nieman2, Renaud F. Warin1 1Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, North Carolina Research Campus, 2Human Performance Laboratory, Appalachian State University, North Carolina Research Campus This work describes an advanced workflow for the accurate and fast determination of NK (Natural Killer) cell count and NK cell cytotoxicity in human blood samples. Immunology and Infection Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood Mary Pat Meaney1, David C. Nieman1, Dru A. Henson2, Qi Jiang3, Fu-Zhang Wang3 1Human Performance Laboratory, North Carolina Research Campus, Appalachian State University, 2Department of Biology, Appalachian State University, 3David H. Murdock Research Institute The purpose of this manuscript is to present a method for measuring monocyte and granulocyte phagocytosis and oxidative burst activity in human blood samples. Medicine Human Skeletal Muscle Biopsy Procedures Using the Modified Bergström Technique R. Andrew Shanely1,2, Kevin A. Zwetsloot2, N. Travis Triplett2, Mary Pat Meaney1,2, Gerard E. Farris3, David C. Nieman1,2 1Human Performance Laboratory, North Carolina Research Campus, Appalacian State University, 2College of Health Sciences, Appalachian State University, 3Department of Emergency Medicine, Carolinas Medical Center NorthEast The purpose of this video is to present the modified Bergström skeletal muscle biopsy technique on human subjects.
