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JoVE Journal Neuroscience
F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes
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F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes

Article DOI: 10.3791/4394-v • 08:21 min • May 4th, 2013
May 4th, 2013 •
1, 1, 1, 1
1Department of Internal Medicine, Yale University

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Summary
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A method to isolate submitochondrial vesicles enriched in F1FO ATP synthase complexes from rat brain is described. These vesicles allow the study of the activity of F1FO ATPase complex and its modulation using the technique of patch clamp recording.

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F1FO ATPase Vesicle Preparation Patch Clamp Recordings Submitochondrial Vesicle Membranes Mitochondria ATP Production Oxidative Phosphorylation Programmed Cell Death F1FO-ATP Synthase Apoptosis Necrosis BCL-2 Family Proteins Mitochondrial Outer Membrane Pro-apoptotic Factors Cytosol Necrotic Cell Death Matrix Calcium Mitochondrial Permeability Transition Pore (mPTP) Inner Membrane Pore Bcl-xL Protein
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