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Cellular Redox Profiling ved hjelp av høy innholdsmikroskopi
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Cellular Redox Profiling ved hjelp av høy innholdsmikroskopi

Article DOI: 10.3791/55449-v • 11:37 min • May 14th, 2017
May 14th, 2017 •
1,2, 1,2, 1, 3, 1,2
1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

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I dette papiret presenteres en mikroprosess arbeidsflyt med høy innhold for samtidig kvantifisering av intracellulære ROS-nivåer, samt mitokondrielt membranpotensial og morfologi - i fellesskap referert til som mitokondriell morfofunksjon - i levende vedhengende celler ved bruk av celle-permeant fluorescerende reportermolekyler 5- (og- 6) -klorometyl-2 ', 7'-diklorodihydrofluoresceindiacetat, acetylester (CM-H2 DCFDA) og tetrametylrhodamin-metylester (TMRM).

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Cellular Biology Reactive Oxygen Species Mitochondria Mitochondrial Morphofunction Live Cell Imaging High-innhold Mikroskopi Fluorescensmikroskopi Quantitative Multiparametric Microscopy Redox Biology
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