Henning Urlaub Bioanalytical Mass Spectrometry Group Max Planck Institute for Biophysical Chemistry Biography Publications Institution JoVE Articles Henning Urlaub has not added a biography. If you are Henning Urlaub and would like to personalize this page please email our Author Liaison for assistance. Publications FLT3-ITD and TLR9 Employ Bruton's Tyrosine Kinase to Activate Distinct Transcriptional Programs Mediating AML Cell Survival and Proliferation Blood. Jan, 2015 | Pubmed ID: 25605370 The RNA Helicase Aquarius Exhibits Structural Adaptations Mediating Its Recruitment to Spliceosomes Nature Structural & Molecular Biology. Feb, 2015 | Pubmed ID: 25599396 Dual Tagging As an Approach to Isolate Endogenous Chromatin Remodeling Complexes from Saccharomyces Cerevisiae Biochimica Et Biophysica Acta. Mar, 2015 | Pubmed ID: 25486077 Modulation of TCR Responsiveness by the Grb2-family Adaptor, Gads Cellular Signalling. Jan, 2015 | Pubmed ID: 25452106 Studying Macromolecular Complex Stoichiometries by Peptide-based Mass Spectrometry Proteomics. Dec, 2014 | Pubmed ID: 25546807 Effects of Mycophenolate Mofetil on Kidney Function and Phosphorylation Status of Renal Proteins in Alport COL4A3-deficient Mice Proteome Science. 2014 | Pubmed ID: 25525413 Structural Analyses of the CRISPR Protein Csc2 Reveal the RNA-binding Interface of the Type I-D Cas7 Family RNA Biology. Aug, 2014 | Pubmed ID: 25483036 RNA Targeting by the Type III-A CRISPR-Cas Csm Complex of Thermus Thermophilus Molecular Cell. Nov, 2014 | Pubmed ID: 25457165 Photo-cross-linking and High-resolution Mass Spectrometry for Assignment of RNA-binding Sites in RNA-binding Proteins Nature Methods. Oct, 2014 | Pubmed ID: 25173706 Structural Analyses of the CRISPR Protein Csc2 Reveal the RNA-binding Interface of the Type I-D Cas7 Family RNA Biology. Aug, 2014 | Pubmed ID: 25117179 Analyzing the Protein Assembly and Dynamics of the Human Spliceosome with SILAC Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 25059615 Biophysical and Biochemical Analysis of HnRNP K: Arginine Methylation, Reversible Aggregation and Combinatorial Binding to Nucleic Acids Biological Chemistry. Jul, 2014 | Pubmed ID: 25003387 Composition of Isolated Synaptic Boutons Reveals the Amounts of Vesicle Trafficking Proteins Science (New York, N.Y.). May, 2014 | Pubmed ID: 24876496 Separation Methodology to Improve Proteome Coverage Depth Expert Review of Proteomics. Aug, 2014 | Pubmed ID: 24834482 Clathrin Heavy Chain Plays Multiple Roles in Polarizing the Drosophila Oocyte Downstream of Bic-D Development (Cambridge, England). May, 2014 | Pubmed ID: 24718986 The NHL Domain of BRAT is an RNA-binding Domain That Directly Contacts the Hunchback MRNA for Regulation Genes & Development. Apr, 2014 | Pubmed ID: 24696456 In Vitro Assembly and Activity of an Archaeal CRISPR-Cas Type I-A Cascade Interference Complex Nucleic Acids Research. Apr, 2014 | Pubmed ID: 24500198 A Complex of Cas Proteins 5, 6, and 7 is Required for the Biogenesis and Stability of Clustered Regularly Interspaced Short Palindromic Repeats (crispr)-derived Rnas (crrnas) in Haloferax Volcanii The Journal of Biological Chemistry. Mar, 2014 | Pubmed ID: 24459147 Mass Spectrometry-based Relative Quantification of Proteins in Precatalytic and Catalytically Active Spliceosomes by Metabolic Labeling (SILAC), Chemical Labeling (iTRAQ), and Label-free Spectral Count RNA (New York, N.Y.). Mar, 2014 | Pubmed ID: 24448447 Proteomic Screening of Glutamatergic Mouse Brain Synaptosomes Isolated by Fluorescence Activated Sorting The EMBO Journal. Jan, 2014 | Pubmed ID: 24413018 Insights into the Activation of the Helicase Prp43 by Biochemical Studies and Structural Mass Spectrometry Nucleic Acids Research. Jan, 2014 | Pubmed ID: 24165877 Phosphorylation Drives a Dynamic Switch in Serine/arginine-rich Proteins Structure (London, England : 1993). Dec, 2013 | Pubmed ID: 24183573 Cutting Edge: Feed-forward Activation of Phospholipase Cγ2 Via C2 Domain-mediated Binding to SLP65 Journal of Immunology (Baltimore, Md. : 1950). Dec, 2013 | Pubmed ID: 24166973 Structure and RNA-binding Properties of the Not1-Not2-Not5 Module of the Yeast Ccr4-Not Complex Nature Structural & Molecular Biology. Nov, 2013 | Pubmed ID: 24121231 Immunostimulatory Activity of Murine Keratinocyte-derived Exosomes Experimental Dermatology. Oct, 2013 | Pubmed ID: 24079734 The SUMO1-E67 Interacting Loop Peptide is an Allosteric Inhibitor of the Dipeptidyl Peptidases 8 and 9 The Journal of Biological Chemistry. Nov, 2013 | Pubmed ID: 24072711 Differential Roles of Postsynaptic Density-93 Isoforms in Regulating Synaptic Transmission The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Sep, 2013 | Pubmed ID: 24068818 The Heme a Synthase Cox15 Associates with Cytochrome C Oxidase Assembly Intermediates During Cox1 Maturation Molecular and Cellular Biology. Oct, 2013 | Pubmed ID: 23979592 Increased Proteome Coverage by Combining PAGE and Peptide Isoelectric Focusing: Comparative Study of Gel-based Separation Approaches Proteomics. Oct, 2013 | Pubmed ID: 23943586 Systematic Identification of Proteins Binding to Chromatin-embedded Ubiquitylated H2B Reveals Recruitment of SWI/SNF to Regulate Transcription Cell Reports. Aug, 2013 | Pubmed ID: 23933260 Structural Analysis of the Yeast Dhh1-Pat1 Complex Reveals How Dhh1 Engages Pat1, Edc3 and RNA in Mutually Exclusive Interactions Nucleic Acids Research. Sep, 2013 | Pubmed ID: 23851565 Choroid Plexus Transcytosis and Exosome Shuttling Deliver Folate into Brain Parenchyma Nature Communications. 2013 | Pubmed ID: 23828504 The Nuclear F-actin Interactome of Xenopus Oocytes Reveals an Actin-bundling Kinesin That is Essential for Meiotic Cytokinesis The EMBO Journal. Jul, 2013 | Pubmed ID: 23727888 Mover is a Homomeric Phospho-protein Present on Synaptic Vesicles PloS One. 2013 | Pubmed ID: 23723986 Molecular Profiling of Synaptic Vesicle Docking Sites Reveals Novel Proteins but Few Differences Between Glutamatergic and GABAergic Synapses Neuron. Apr, 2013 | Pubmed ID: 23622064 Anle138b: a Novel Oligomer Modulator for Disease-modifying Therapy of Neurodegenerative Diseases Such As Prion and Parkinson's Disease Acta Neuropathologica. Jun, 2013 | Pubmed ID: 23604588 β2 Integrin-derived Signals Induce Cell Survival and Proliferation of AML Blasts by Activating a Syk/STAT Signaling Axis Blood. May, 2013 | Pubmed ID: 23509157 Detecting Endogenous SUMO Targets in Mammalian Cells and Tissues Nature Structural & Molecular Biology. Apr, 2013 | Pubmed ID: 23503365 An Automated In-gel Digestion/iTRAQ-labeling Workflow for Robust Quantification of Gel-separated Proteins Proteomics. May, 2013 | Pubmed ID: 23456960 Mitochondrial SIRT4-type Proteins in Caenorhabditis Elegans and Mammals Interact with Pyruvate Carboxylase and Other Acetylated Biotin-dependent Carboxylases Mitochondrion. Nov, 2013 | Pubmed ID: 23438705 Dissection of the Factor Requirements for Spliceosome Disassembly and the Elucidation of Its Dissociation Products Using a Purified Splicing System Genes & Development. Feb, 2013 | Pubmed ID: 23431055 Mass Spectrometry Imaging: Linking Molecule Profiles to Tissue Spatial Distribution Expert Review of Proteomics. Feb, 2013 | Pubmed ID: 23414355 Evolution of the Protein Stoichiometry in the L12 Stalk of Bacterial and Organellar Ribosomes Nature Communications. 2013 | Pubmed ID: 23340427 SCFFbxw5 Mediates Transient Degradation of Actin Remodeller Eps8 to Allow Proper Mitotic Progression Nature Cell Biology. Feb, 2013 | Pubmed ID: 23314863 Identification of DEAD-box RNA Helicase 6 (DDX6) As a Cellular Modulator of Vascular Endothelial Growth Factor Expression Under Hypoxia The Journal of Biological Chemistry. Feb, 2013 | Pubmed ID: 23293030 Identification of CRM1-dependent Nuclear Export Cargos Using Quantitative Mass Spectrometry Molecular & Cellular Proteomics : MCP. Mar, 2013 | Pubmed ID: 23242554 EF-P is Essential for Rapid Synthesis of Proteins Containing Consecutive Proline Residues Science (New York, N.Y.). Jan, 2013 | Pubmed ID: 23239624 The Dok-3/Grb2 Protein Signal Module Attenuates Lyn Kinase-dependent Activation of Syk Kinase in B Cell Antigen Receptor Microclusters The Journal of Biological Chemistry. Jan, 2013 | Pubmed ID: 23223229 Burial of the Polymorphic Residue 129 in Amyloid Fibrils of Prion Stop Mutants The Journal of Biological Chemistry. Feb, 2013 | Pubmed ID: 23209282 Systematic Analysis of Barrier-forming FG Hydrogels from Xenopus Nuclear Pore Complexes The EMBO Journal. Jan, 2013 | Pubmed ID: 23202855 Identification and Characterization of FAM124B As a Novel Component of a CHD7 and CHD8 Containing Complex PloS One. 2012 | Pubmed ID: 23285124 In Vivo Localization and Identification of SUMOylated Proteins in the Brain of His6-HA-SUMO1 Knock-in Mice Proceedings of the National Academy of Sciences of the United States of America. Dec, 2012 | Pubmed ID: 23213215 The Human Cytomegalovirus DNA Polymerase Processivity Factor UL44 is Modified by SUMO in a DNA-dependent Manner PloS One. 2012 | Pubmed ID: 23166733 The Prp8 RNase H-like Domain Inhibits Brr2-mediated U4/U6 SnRNA Unwinding by Blocking Brr2 Loading Onto the U4 SnRNA Genes & Development. Nov, 2012 | Pubmed ID: 23124066 The Claudin Megatrachea Protein Complex The Journal of Biological Chemistry. Oct, 2012 | Pubmed ID: 22930751 Ubiquitin-specific Protease-like 1 (USPL1) is a SUMO Isopeptidase with Essential, Non-catalytic Functions EMBO Reports. Oct, 2012 | Pubmed ID: 22878415 Post-transcriptional Spliceosomes Are Retained in Nuclear Speckles Until Splicing Completion Nature Communications. 2012 | Pubmed ID: 22871813 Biophysical Properties and Cellular Toxicity of Covalent Crosslinked Oligomers of α-synuclein Formed by Photoinduced Side-chain Tyrosyl Radicals Free Radical Biology & Medicine. Aug, 2012 | Pubmed ID: 22771470 Structure and Activity of the Only Human RNase T2 Nucleic Acids Research. Sep, 2012 | Pubmed ID: 22735700 Determinants of Interaction Specificity of the Bacillus Subtilis GlcT Antitermination Protein: Functionality and Phosphorylation Specificity Depend on the Arrangement of the Regulatory Domains The Journal of Biological Chemistry. Aug, 2012 | Pubmed ID: 22722928 Identification of Endogenous SUMO1 Accepter Sites by Mass Spectrometry Methods in Molecular Biology (Clifton, N.J.). 2012 | Pubmed ID: 22665316 Absolute Quantification of Proteins Using Standard Peptides and Multiple Reaction Monitoring Methods in Molecular Biology (Clifton, N.J.). 2012 | Pubmed ID: 22665306 Quantitative Mass Spectrometry-based Proteomics: an Overview Methods in Molecular Biology (Clifton, N.J.). 2012 | Pubmed ID: 22665296 Investigation of Protein-RNA Interactions by Mass Spectrometry--Techniques and Applications Journal of Proteomics. Jun, 2012 | Pubmed ID: 22575267 The Guanine Nucleotide Exchange Factor Vav2 is a Negative Regulator of Parathyroid Hormone Receptor/Gq Signaling Molecular Pharmacology. Aug, 2012 | Pubmed ID: 22554804 The Cryo-EM Structure of the UPF-EJC Complex Shows UPF1 Poised Toward the RNA 3' End Nature Structural & Molecular Biology. May, 2012 | Pubmed ID: 22522823 OKB, a Novel Family of Brain-gut Neuropeptides from Insects Insect Biochemistry and Molecular Biology. Jul, 2012 | Pubmed ID: 22480496 Crystal Structure of Cwc2 Reveals a Novel Architecture of a Multipartite RNA-binding Protein The EMBO Journal. May, 2012 | Pubmed ID: 22407296 Late Motor Decline After Accomplished Remyelination: Impact for Progressive Multiple Sclerosis Annals of Neurology. Feb, 2012 | Pubmed ID: 22367995 Syk Kinase-coupled C-type Lectin Receptors Engage Protein Kinase C-σ to Elicit Card9 Adaptor-mediated Innate Immunity Immunity. Jan, 2012 | Pubmed ID: 22265677 BC1-FMRP Interaction is Modulated by 2'-O-methylation: RNA-binding Activity of the Tudor Domain and Translational Regulation at Synapses Nucleic Acids Research. May, 2012 | Pubmed ID: 22238374 MINOS1 is a Conserved Component of Mitofilin Complexes and Required for Mitochondrial Function and Cristae Organization Molecular Biology of the Cell. Jan, 2012 | Pubmed ID: 22114354 Phosphorylation Site Localization in Peptides by MALDI MS/MS and the Mascot Delta Score Analytical and Bioanalytical Chemistry. Jan, 2012 | Pubmed ID: 22038583 Optic Atrophy 1 is an A-kinase Anchoring Protein on Lipid Droplets That Mediates Adrenergic Control of Lipolysis The EMBO Journal. Nov, 2011 | Pubmed ID: 21983901 A Reversibly Photoswitchable GFP-like Protein with Fluorescence Excitation Decoupled from Switching Nature Biotechnology. Oct, 2011 | Pubmed ID: 21909082 Chromatin Affinity Purification and Quantitative Mass Spectrometry Defining the Interactome of Histone Modification Patterns Molecular & Cellular Proteomics : MCP. Nov, 2011 | Pubmed ID: 21836164 The B-cell Antigen Receptor Signals Through a Preformed Transducer Module of SLP65 and CIN85 The EMBO Journal. Aug, 2011 | Pubmed ID: 21822214 Pabp Binds to the Osk 3'UTR and Specifically Contributes to Osk MRNA Stability and Oocyte Accumulation Developmental Biology. Sep, 2011 | Pubmed ID: 21782810 Sumoylation Inhibits Alpha-synuclein Aggregation and Toxicity The Journal of Cell Biology. Jul, 2011 | Pubmed ID: 21746851 Sequential Phosphorylation of SLP-76 at Tyrosine 173 is Required for Activation of T and Mast Cells The EMBO Journal. Aug, 2011 | Pubmed ID: 21725281 B Cell Receptor-mediated Antigen Gathering Requires Ubiquitin Ligase Cbl and Adaptors Grb2 and Dok-3 to Recruit Dynein to the Signaling Microcluster Immunity. Jun, 2011 | Pubmed ID: 21703542 Functional Proteomics of Neuropeptidome Dynamics During the Feeding Process of Rhodnius Prolixus Journal of Proteome Research. Aug, 2011 | Pubmed ID: 21699238 Semiquantitative Proteomic Analysis of the Human Spliceosome Via a Novel Two-dimensional Gel Electrophoresis Method Molecular and Cellular Biology. Jul, 2011 | Pubmed ID: 21536652 A Cytoplasmic Complex Mediates Specific MRNA Recognition and Localization in Yeast PLoS Biology. Apr, 2011 | Pubmed ID: 21526221 Complex Phosphorylation Dynamics Control the Composition of the Syk Interactome in B Cells European Journal of Immunology. Jun, 2011 | Pubmed ID: 21469132 RACK1 is a Novel Interaction Partner of PTK7 That is Required for Neural Tube Closure Development (Cambridge, England). Apr, 2011 | Pubmed ID: 21350015 Fc Gamma Receptor IIb Modulates the Molecular Grb2 Interaction Network in Activated B Cells Cellular Signalling. May, 2011 | Pubmed ID: 21262349 Synthesis and Characterization of Pseudocantharidins, Novel Phosphatase Modulators That Promote the Inclusion of Exon 7 into the SMN (survival of Motoneuron) Pre-mRNA The Journal of Biological Chemistry. Mar, 2011 | Pubmed ID: 21220421 7SK Small Nuclear RNA Directly Affects HMGA1 Function in Transcription Regulation Nucleic Acids Research. Mar, 2011 | Pubmed ID: 21087998 Phosphorylation of Human Argonaute Proteins Affects Small RNA Binding Nucleic Acids Research. Mar, 2011 | Pubmed ID: 21071408 The Human Pat1b Protein: a Novel MRNA Deadenylation Factor Identified by a New Immunoprecipitation Technique Nucleic Acids Research. Jan, 2011 | Pubmed ID: 20852261 PERILIPIN-dependent Control of Lipid Droplet Structure and Fat Storage in Drosophila Cell Metabolism. Nov, 2010 | Pubmed ID: 21035762 Characterization of Purified Human Bact Spliceosomal Complexes Reveals Compositional and Morphological Changes During Spliceosome Activation and First Step Catalysis RNA (New York, N.Y.). Dec, 2010 | Pubmed ID: 20980672 A Novel Nop5-sRNA Interaction That is Required for Efficient Archaeal Box C/D SRNP Formation RNA (New York, N.Y.). Dec, 2010 | Pubmed ID: 20962039 Quantitative Analysis of Synaptic Vesicle Rabs Uncovers Distinct Yet Overlapping Roles for Rab3a and Rab27b in Ca2+-triggered Exocytosis The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Oct, 2010 | Pubmed ID: 20926670 Pseudo-neutral-loss Scan for Selective Detection of Phosphopeptides and N-glycopeptides Using Liquid Chromatography Coupled with a Hybrid Linear Ion-trap/orbitrap Mass Spectrometer Proteomics. Nov, 2010 | Pubmed ID: 20925059 DDX6 Recruits Translational Silenced Human Reticulocyte 15-lipoxygenase MRNA to RNP Granules RNA (New York, N.Y.). Nov, 2010 | Pubmed ID: 20884783 The Archaeal Lsm Protein Binds to Small RNAs The Journal of Biological Chemistry. Nov, 2010 | Pubmed ID: 20826804 Merging Molecular Electron Microscopy and Mass Spectrometry by Carbon Film-assisted Endoproteinase Digestion Molecular & Cellular Proteomics : MCP. Aug, 2010 | Pubmed ID: 20530635 Mapping the Binding Site of Snurportin 1 on Native U1 SnRNP by Cross-linking and Mass Spectrometry Nucleic Acids Research. Sep, 2010 | Pubmed ID: 20421206 Exon Definition Complexes Contain the Tri-snRNP and Can Be Directly Converted into B-like Precatalytic Splicing Complexes Molecular Cell. Apr, 2010 | Pubmed ID: 20417601 Extraction of Proteins from Formalin-fixed, Paraffin-embedded Tissue Using the Qproteome Extraction Technique and Preparation of Tryptic Peptides for Liquid Chromatography/mass Spectrometry Analysis Current Protocols in Molecular Biology / Edited by Frederick M. Ausubel ... [et Al.]. Apr, 2010 | Pubmed ID: 20373500 Determination of Protein Stoichiometry Within Protein Complexes Using Absolute Quantification and Multiple Reaction Monitoring Analytical Chemistry. Apr, 2010 | Pubmed ID: 20225856 A Syd-1 Homologue Regulates Pre- and Postsynaptic Maturation in Drosophila The Journal of Cell Biology. Feb, 2010 | Pubmed ID: 20176924 Molecular Architecture of the Human Prp19/CDC5L Complex Molecular and Cellular Biology. May, 2010 | Pubmed ID: 20176811 Human PRP4 Kinase is Required for Stable Tri-snRNP Association During Spliceosomal B Complex Formation Nature Structural & Molecular Biology. Feb, 2010 | Pubmed ID: 20118938 Crystal Structure of an Intramolecular Chaperone Mediating Triple-beta-helix Folding Nature Structural & Molecular Biology. Feb, 2010 | Pubmed ID: 20118935 Essential Role of a Trypanosome U4-specific Sm Core Protein in Small Nuclear Ribonucleoprotein Assembly and Splicing Eukaryotic Cell. Mar, 2010 | Pubmed ID: 20081062 Quantitative Comparison of Glutamatergic and GABAergic Synaptic Vesicles Unveils Selectivity for Few Proteins Including MAL2, a Novel Synaptic Vesicle Protein The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jan, 2010 | Pubmed ID: 20053882 The Evolutionarily Conserved Core Design of the Catalytic Activation Step of the Yeast Spliceosome Molecular Cell. Nov, 2009 | Pubmed ID: 19941820 Reconstitution of Both Steps of Saccharomyces Cerevisiae Splicing with Purified Spliceosomal Components Nature Structural & Molecular Biology. Dec, 2009 | Pubmed ID: 19935684 Proteomic Basics - Quantification and Post-translational Modifications of Proteins: The 3rd European Summer School in Kloster Neustift Journal of Proteomics. Nov, 2009 | Pubmed ID: 19931431 The B-lymphoid Grb2 Interaction Code Immunological Reviews. Nov, 2009 | Pubmed ID: 19909361 A New Paradigm for MAPK: Structural Interactions of HERK1 with Mitochondria in HeLa Cells PloS One. 2009 | Pubmed ID: 19847302 Multimerization and H3K9me3 Binding Are Required for CDYL1b Heterochromatin Association The Journal of Biological Chemistry. Dec, 2009 | Pubmed ID: 19808672 In Vivo Expression and Purification of Aptamer-tagged Small RNA Regulators Nucleic Acids Research. Nov, 2009 | Pubmed ID: 19726584 "ChopNSpice," a Mass Spectrometric Approach That Allows Identification of Endogenous Small Ubiquitin-like Modifier-conjugated Peptides Molecular & Cellular Proteomics : MCP. Dec, 2009 | Pubmed ID: 19721078 Initial Insight into the Function of the Lysosomal 66.3 KDa Protein from Mouse by Means of X-ray Crystallography BMC Structural Biology. 2009 | Pubmed ID: 19706171 The Cytoplasmic Peptidase DPP9 is Rate-limiting for Degradation of Proline-containing Peptides The Journal of Biological Chemistry. Oct, 2009 | Pubmed ID: 19667070 SMN-assisted Assembly of SnRNP-specific Sm Cores in Trypanosomes Genes & Development. Jul, 2009 | Pubmed ID: 19605687 ITRAQ-labeling of In-gel Digested Proteins for Relative Quantification Methods in Molecular Biology (Clifton, N.J.). 2009 | Pubmed ID: 19544025 SLP-65 Phosphorylation Dynamics Reveals a Functional Basis for Signal Integration by Receptor-proximal Adaptor Proteins Molecular & Cellular Proteomics : MCP. Jul, 2009 | Pubmed ID: 19372136 Importin 8 is a Gene Silencing Factor That Targets Argonaute Proteins to Distinct MRNAs Cell. Feb, 2009 | Pubmed ID: 19167051 Enrichment of Protein-RNA Crosslinks from Crude UV-irradiated Mixtures for MS Analysis by On-line Chromatography Using Titanium Dioxide Columns Biopolymers. Apr, 2009 | Pubmed ID: 19140157 The Neuropeptidome of Rhodnius Prolixus Brain Proteomics. Feb, 2009 | Pubmed ID: 19137558 Atmospheric Pressure Free Liquid Infrared MALDI Mass Spectrometry: Toward a Combined ESI/ MALDI-liquid Chromatography Interface Analytical Chemistry. Jan, 2009 | Pubmed ID: 19125446 SAP18 Promotes Krüppel-dependent Transcriptional Repression by Enhancer-specific Histone Deacetylation The Journal of Biological Chemistry. Jan, 2009 | Pubmed ID: 19049982 Structure of Tripeptidyl-peptidase I Provides Insight into the Molecular Basis of Late Infantile Neuronal Ceroid Lipofuscinosis The Journal of Biological Chemistry. Feb, 2009 | Pubmed ID: 19038966 Stalling of Spliceosome Assembly at Distinct Stages by Small-molecule Inhibitors of Protein Acetylation and Deacetylation RNA (New York, N.Y.). Jan, 2009 | Pubmed ID: 19029308 Conservation of the Protein Composition and Electron Microscopy Structure of Drosophila Melanogaster and Human Spliceosomal Complexes Molecular and Cellular Biology. Jan, 2009 | Pubmed ID: 18981222 Structural and Functional Analysis of the E. Coli NusB-S10 Transcription Antitermination Complex Molecular Cell. Dec, 2008 | Pubmed ID: 19111659 Analyzing RNA-protein Crosslinking Sites in Unlabeled Ribonucleoprotein Complexes by Mass Spectrometry Methods in Molecular Biology (Clifton, N.J.). 2008 | Pubmed ID: 18982295 Mechanism and Consequences for Paralog-specific Sumoylation of Ubiquitin-specific Protease 25 Molecular Cell. Jun, 2008 | Pubmed ID: 18538659 Phosphorylation of Human PRP28 by SRPK2 is Required for Integration of the U4/U6-U5 Tri-snRNP into the Spliceosome Nature Structural & Molecular Biology. May, 2008 | Pubmed ID: 18425142 CASK Functions As a Mg2+-independent Neurexin Kinase Cell. Apr, 2008 | Pubmed ID: 18423203 Isolation of an Active Step I Spliceosome and Composition of Its RNP Core Nature. Apr, 2008 | Pubmed ID: 18322460 Spatial Interplay Between PIASy and FIP200 in the Regulation of Signal Transduction and Transcriptional Activity Molecular and Cellular Biology. Apr, 2008 | Pubmed ID: 18285457 'Proteomic Basics--sample Preparation and Separation': the 1st European Summer School in Kloster Neustift, 12-18 August, 2007 Brixen/Bressanone, South Tyrol, Italy Proteomics. Jan, 2008 | Pubmed ID: 18203264 GraFix: Sample Preparation for Single-particle Electron Cryomicroscopy Nature Methods. Jan, 2008 | Pubmed ID: 18157137 Two DEAD-box Proteins May Be Part of RNA-dependent High-molecular-mass Protein Complexes in Arabidopsis Mitochondria Plant Physiology. Dec, 2007 | Pubmed ID: 17951454 Proteomic and Functional Analysis of Argonaute-containing MRNA-protein Complexes in Human Cells EMBO Reports. Nov, 2007 | Pubmed ID: 17932509 Improved Identification of Enriched Peptide RNA Cross-links from Ribonucleoprotein Particles (RNPs) by Mass Spectrometry Nucleic Acids Research. 2007 | Pubmed ID: 17652325 Role of Cdk2 in the Sequential Phosphorylation/activation of C/EBPbeta During Adipocyte Differentiation Proceedings of the National Academy of Sciences of the United States of America. Jul, 2007 | Pubmed ID: 17601773 In Vitro RNA Editing in Plant Mitochondria Does Not Require Added Energy FEBS Letters. Jun, 2007 | Pubmed ID: 17531229 Detection of Protein-RNA Crosslinks by NanoLC-ESI-MS/MS Using Precursor Ion Scanning and Multiple Reaction Monitoring (MRM) Experiments Journal of the American Society for Mass Spectrometry. May, 2007 | Pubmed ID: 17349801 Composition and Three-dimensional EM Structure of Double Affinity-purified, Human Prespliceosomal A Complexes The EMBO Journal. Mar, 2007 | Pubmed ID: 17332742 Functional Interaction Between PML and SATB1 Regulates Chromatin-loop Architecture and Transcription of the MHC Class I Locus Nature Cell Biology. Jan, 2007 | Pubmed ID: 17173041 Protein Composition of Human MRNPs Spliced in Vitro and Differential Requirements for MRNP Protein Recruitment RNA (New York, N.Y.). Jan, 2007 | Pubmed ID: 17095540 Molecular Anatomy of a Trafficking Organelle Cell. Nov, 2006 | Pubmed ID: 17110340 The Methanothermobacter Thermautotrophicus ExoIII Homologue Mth212 is a DNA Uridine Endonuclease Nucleic Acids Research. 2006 | Pubmed ID: 17012282 Protein Composition and Electron Microscopy Structure of Affinity-purified Human Spliceosomal B Complexes Isolated Under Physiological Conditions Molecular and Cellular Biology. Jul, 2006 | Pubmed ID: 16809785 Identification, Cloning, and Functional Analysis of the Human U6 SnRNA-specific Terminal Uridylyl Transferase RNA (New York, N.Y.). Aug, 2006 | Pubmed ID: 16790842 Identification of SNAP-47, a Novel Qbc-SNARE with Ubiquitous Expression The Journal of Biological Chemistry. Jun, 2006 | Pubmed ID: 16621800 RNA Structure and RNA-protein Interactions in Purified Yeast U6 SnRNPs Journal of Molecular Biology. Mar, 2006 | Pubmed ID: 16410014 Complete MALDI-ToF MS Analysis of Cross-linked Peptide-RNA Oligonucleotides Derived from Nonlabeled UV-irradiated Ribonucleoprotein Particles RNA (New York, N.Y.). Dec, 2005 | Pubmed ID: 16314460 Identification of Novel Argonaute-associated Proteins Current Biology : CB. Dec, 2005 | Pubmed ID: 16289642 Fragile X Mental Retardation Protein (FMRP) Binds Specifically to the Brain Cytoplasmic RNAs BC1/BC200 Via a Novel RNA-binding Motif The Journal of Biological Chemistry. Sep, 2005 | Pubmed ID: 16006558 Functional Analysis of Seven Genes Encoding Eight Translation Initiation Factor 4E (eIF4E) Isoforms in Drosophila Mechanisms of Development. Apr, 2005 | Pubmed ID: 15804566 Proteomic Analysis of Reaper 5' Untranslated Region-interacting Factors Isolated by Tobramycin Affinity-selection Reveals a Role for La Antigen in Reaper MRNA Translation Proteomics. Apr, 2005 | Pubmed ID: 15789343 Characterization of the Cleavage Site and Function of Resulting Cleavage Fragments After Limited Proteolysis of Clostridium Difficile Toxin B (TcdB) by Host Cells Microbiology (Reading, England). Jan, 2005 | Pubmed ID: 15632438 Assembly and Maturation of the U3 SnoRNP in the Nucleoplasm in a Large Dynamic Multiprotein Complex Molecular Cell. Dec, 2004 | Pubmed ID: 15574333 Purification, Crystallization and Preliminary Crystallographic Data of the M3G Cap-binding Domain of Human SnRNP Import Factor Snurportin 1 Acta Crystallographica. Section D, Biological Crystallography. Sep, 2004 | Pubmed ID: 15333938 Crystal Structures of the Antitermination Factor NusB from Thermotoga Maritima and Implications for RNA Binding The Biochemical Journal. Nov, 2004 | Pubmed ID: 15279620 A Subset of Human 35S U5 Proteins, Including Prp19, Function Prior to Catalytic Step 1 of Splicing The EMBO Journal. Jun, 2004 | Pubmed ID: 15175653 The Human 18S U11/U12 SnRNP Contains a Set of Novel Proteins Not Found in the U2-dependent Spliceosome RNA (New York, N.Y.). Jun, 2004 | Pubmed ID: 15146077 Analysis of Site-specific Protein-RNA Cross-links in Isolated RNP Complexes, Combining Affinity Selection and Mass Spectrometry RNA (New York, N.Y.). Dec, 2003 | Pubmed ID: 14624009 The Zinc Finger-associated Domain of the Drosophila Transcription Factor Grauzone is a Novel Zinc-coordinating Protein-protein Interaction Module Structure (London, England : 1993). Nov, 2003 | Pubmed ID: 14604529 Analysis of Mammalian Gene Function Using Small Interfering RNAs Nucleic Acids Research. Supplement (2001). 2003 | Pubmed ID: 14510516 Mutagenesis Suggests Several Roles of Snu114p in Pre-mRNA Splicing The Journal of Biological Chemistry. Jul, 2003 | Pubmed ID: 12736260 An Unusual Mechanism of Bacterial Gene Expression Revealed for the RNase P Protein of Thermus Strains Proceedings of the National Academy of Sciences of the United States of America. May, 2003 | Pubmed ID: 12719542 Protein Composition of Human Prespliceosomes Isolated by a Tobramycin Affinity-selection Method Proceedings of the National Academy of Sciences of the United States of America. Dec, 2002 | Pubmed ID: 12477934 Small Nuclear Ribonucleoprotein Remodeling During Catalytic Activation of the Spliceosome Science (New York, N.Y.). Dec, 2002 | Pubmed ID: 12411573 Hierarchical, Clustered Protein Interactions with U4/U6 SnRNA: a Biochemical Role for U4/U6 Proteins The EMBO Journal. Oct, 2002 | Pubmed ID: 12374753 Characterization of Novel SF3b and 17S U2 SnRNP Proteins, Including a Human Prp5p Homologue and an SF3b DEAD-box Protein The EMBO Journal. Sep, 2002 | Pubmed ID: 12234937 Single-stranded Antisense SiRNAs Guide Target RNA Cleavage in RNAi Cell. Sep, 2002 | Pubmed ID: 12230974 A Two-tracked Approach to Analyze RNA-protein Crosslinking Sites in Native, Nonlabeled Small Nuclear Ribonucleoprotein Particles Methods (San Diego, Calif.). Feb, 2002 | Pubmed ID: 12054894 Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors Hanibal Bohnenberger1, Philipp Ströbel1, Sebastian Mohr2, Jasmin Corso3, Tobias Berg2, Henning Urlaub3,4, Christof Lenz3,4, Hubert Serve2,5,6, Thomas Oellerich2,5,6 1Institute of Pathology, University Medical Center, Göttingen, 2Department of Hematology/Oncology, Goethe University of Frankfurt, 3Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 4Bioanalytics, Institute of Clinical Chemistry, University Medical Center, Göttingen, 5German Cancer Consortium, 6German Cancer Research Center JoVE 52435 Medicine
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors Hanibal Bohnenberger1, Philipp Ströbel1, Sebastian Mohr2, Jasmin Corso3, Tobias Berg2, Henning Urlaub3,4, Christof Lenz3,4, Hubert Serve2,5,6, Thomas Oellerich2,5,6 1Institute of Pathology, University Medical Center, Göttingen, 2Department of Hematology/Oncology, Goethe University of Frankfurt, 3Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 4Bioanalytics, Institute of Clinical Chemistry, University Medical Center, Göttingen, 5German Cancer Consortium, 6German Cancer Research Center JoVE 52435 Medicine