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JoVE Journal Biology
Metabolsk Merking av ny-transkriberte RNA for High Resolution Gene Expression Profilering av RNA syntese, bearbeiding og Decay i cellekultur
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Metabolsk Merking av ny-transkriberte RNA for High Resolution Gene Expression Profilering av RNA syntese, bearbeiding og Decay i cellekultur

Article DOI: 10.3791/50195-v • 11:00 min • August 8th, 2013
August 8th, 2013 •
*1, *2, 1, 3, 1, 2
1Max von Pettenkofer Institute, 2Department of Medicine, University of Cambridge, 3Institute for Informatics, Ludwig-Maximilians-University Munich
* These authors contributed equally

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Total cellulær RNA gir et dårlig mal for å studere kortvarige endringer i RNA-syntese og nedbrytning, så vel som kinetikken til RNA prosessering. Her beskriver vi metabolsk merking av nylig transkriberte RNA med 4-thiouridine etterfulgt av tiol-spesifikk biotinylering og rensing av nylig transkriberte RNA tillater å overvinne disse begrensningene.

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Genetikk cellebiologi molekylær biologi mikrobiologi biokjemi Eukaryota etterforskningsmetoder Biologiske Phenomena Gene expression profilering RNA syntese RNA prosessering RNA forfall 4-thiouridine 4SU-tagging microarray analyse RNA-seq RNA DNA PCR sekvensering
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