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JoVE Journal Biology
Luminométricos levedura e
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Luminométricos levedura e

Article DOI: 10.3791/50816-v • 12:09 min • December 31st, 2013
December 31st, 2013 •
1, 1, 1, 1,2
1Laboratory of Cell and Molecular Biology, University of Wisconsin – Madison, 2Department of Genetics, University of Wisconsin – Madison

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Para complementar os métodos habitualmente utilizados para estudar TRPV4 de, dois métodos são descritos: O mecanosensibilidade pode ser estudada por Ca 2 +-aequorina luminometria em leveduras transgénicas após desafio com hipo-osmótico. Ele também pode ser examinada em TRPV4-ARN injectado oócitos de Xenopus a-célula inteira de dois eléctrodos de aperto de voltagem ou remendo grampo em no modo de célula ou excisadas.

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Protocolo Básico Edição 82 Eukaryota Archaea bactérias as Ciências da Vida (Geral) Mechanosensation canais iônicos Lipídeos patch clamp, fermento luminometria força de sensoriamento braçadeira de tensão TRPV4 eletrofisiologia
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