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JoVE Journal
Neuroscience

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通过激光捕获微分路,从小鼠脊髓运动神经细胞体中生成RNA进行转录分析
 
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通过激光捕获微分路,从小鼠脊髓运动神经细胞体中生成RNA进行转录分析

Article DOI: 10.3791/51168-v 11:46 min January 13th, 2014
January 13th, 2014

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Summary

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Click here for the English version.

通过激光捕捉微分,用 Azure B 染色 70% 乙醇的脊髓部分后,从小鼠脊髓运动神经元的细胞体中制备了高质量的总RNA。从 3,000-4,000 个运动神经元中恢复足够的 RNA(+40-60 ng),以便通过 RNA-seq 和 qRT-PCR 进行下游 RNA 分析。

Tags

神经科学,第83期,激光捕捉微分,运动神经元,脊髓,蔚蓝B,RNA,RNA-seq,qRT-PCR
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