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Immunology and Infection

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JoVE Journal Immunology and Infection
Transformação Biolistic de uma fluorescente Tagged Gene para o oportunista fúngica Pathogen
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Transformação Biolistic de uma fluorescente Tagged Gene para o oportunista fúngica Pathogen

Article DOI: 10.3791/52666-v • 07:32 min • March 19th, 2015
March 19th, 2015 •
1, 2, 1, 1
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC), Clemson University, 2Department of Biology, Western Carolina University

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Biolistic transformação é um método usado para gerar a integração estável de ADN no genoma das oportunistas Cryptococcus neoformans patogénio através de recombinação homóloga. Iremos demonstrar transformação biolística de uma construção, o qual tem o gene que codifica a quinase de etilo fundido com o marcador fluorescente mCherry em C. neoformans.

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Biologia Molecular Edição 97 disrupção do gene, Entrega biolística cinase de etilo sobreposição de PCR a fluorescência
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