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Dissekera multi signalering proteinkomplex av Bimolecular komplettering affinitet rening (BiCAP)
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Dissekera multi signalering proteinkomplex av Bimolecular komplettering affinitet rening (BiCAP)

Article DOI: 10.3791/57109-v • 06:45 min • June 15th, 2018
June 15th, 2018 •
1, 1, 1,2, 1,3, 1,4, 5, 1,6,7
1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3RCSI Molecular Medicine, Royal College of Surgeons in Ireland, 4Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences, University of New South Wales, 6St Vincent's Hospital Clinical School, University of New South Wales, 7School of Medicine and Medical Science, University College Dublin

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Detta manuskript beskriver protokollet för Bimolecular komplettering affinitet rening (BiCAP). Denna nya metod underlättar specifika isolering och nedströms proteomiska karakterisering av några två samverkande proteiner, medan exklusive FN-komplex enskilda proteiner samt komplex bildas med konkurrerande bindande partner.

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Immunologi och infektion fråga 136 Protein-protein interaktioner proteomik Interactomics BiCAP signaltransduktion Bimolecular fluorescens komplettering Nanobody
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