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Journal / Immunology and Infection / Imagen tridimensional da Vasculatura e Drenagem…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Imagen tridimensional da Vasculatura e Drenagem Linfática Vertebral usando iDISCO+ e Microscopia de Fluorescência de Folha de Luz
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Imagen tridimensional da Vasculatura e Drenagem Linfática Vertebral usando iDISCO+ e Microscopia de Fluorescência de Folha de Luz

Article DOI: 10.3791/61099-v • 10:05 min • May 22nd, 2020
May 22nd, 2020 •
*1, *1,2, 1,3
1Université Pierre et Marie Curie Paris, Sorbonne Université, Institut du Cerveau et de la Moelle Epinière, 2Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 3Department of Neurology, Yale University School of Medicine
* These authors contributed equally

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Um protocolo é apresentado combinando limpeza tecidual com microscopia de fluorescência de folha de luz (LSFM) para obter imagens tridimensionais e de resolução celular dos vasos linfáticos e linfonodos (LNs) coletando o fluido cefalorraquidal (CSF) e fluido peridural espinhal.

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Imunologia e Infecção Questão 159 vasculatura linfática meningeal e vertebral linfonodos drenagem iDISCO+ microscópio de fluorescência da folha de luz sistema nervoso central
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