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JoVE Journal Biochemistry
Fosfoproteomisk strategi for profilering av osmotisk stresssignalering i Arabidopsis
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Fosfoproteomisk strategi for profilering av osmotisk stresssignalering i Arabidopsis

Article DOI: 10.3791/61489-v • 05:47 min • June 25th, 2020
June 25th, 2020 •
1,3, 2, 3,4, 5
1Department of Plant Biology, Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences, Kyoto University, 3Department of Biochemistry, Purdue University, 4Department of Chemistry, Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences

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Presentert her er en fosfoproteomisk tilnærming, nemlig stoppe og gå ekstraksjon tips basert fosfoproteomisk, som gir høy gjennomstrømning og dyp dekning av Arabidopsis fosfoproteom. Denne tilnærmingen avgrenser oversikten over osmotisk stresssignalering i Arabidopsis.

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Biokjemi Utgave 160 massespektrometri fosfoproteomics proteinfosforylering scenen tips tilnærming Tandem Mass Tag merking Arabidopsis osmotisk stress
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