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Journal / Immunology and Infection / M1 ve M2 İnsan Monosit Türevi Hücrelerin…
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
M1 ve M2 İnsan Monosit Türevi Hücrelerin Polarizasyonu ve Mikobakteri Tüberküloz Enfeksiyonu Üzerine Akış Sitometrisi ile Analiz
Reliable and High Efficiency Extraction of Kidney Immune Cells
Journal (Immunology and Infection)
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Advanced Enteroid Model for Studying Host-Pathogen Interactions (Video) | JoVE
Journal (Immunology and Infection)
Advanced Enteroid Model for Studying Host-Pathogen Interactions (Video) | JoVE
 
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M1 ve M2 İnsan Monosit Türevi Hücrelerin Polarizasyonu ve Mikobakteri Tüberküloz Enfeksiyonu Üzerine Akış Sitometrisi ile Analiz

Article DOI: 10.3791/61807-v • 10:43 min • September 18th, 2020
September 18th, 2020 •
1,2, 1, 1, 3, 1, 1,4, 1
1Center for Infectious Medicine (CIM), Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, 2Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, 3Clinical Microbiology, Department of Laboratory Medicine (Labmed), ANA Futura, Karolinska Institutet, 4Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet

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Bu protokol, insan M1 veya M2 polarize makrofajlardaki Mycobacterium tuberculosis enfeksiyonunu, periferik-kan monositlerinin GFP etiketli virülan strain H37Rv ile enfekte olan makrofaj benzeri hücrelere farklılaşmasına dayanan ve seçilen M1/M2 belirteçlerinin ifadesini içeren 10 renkli bir panel kullanılarak akış sitometrisi ile analiz edilen bir yöntem sağlar.

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JoVE'de Bu Ay Sayı 163 makrofaj insan polarizasyon M1/M2 Mycobacterium tuberculosis,enfeksiyon immün yanıt akış sitometrisi
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