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Biochemistry

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JoVE Journal Biochemistry
Untersuchungen von Chaperon-Cochaperon-Interaktionen mit homogenem Bead-based Assay
Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes
Journal (Biochemistry)
Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
Journal (Biochemistry)
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
 
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Untersuchungen von Chaperon-Cochaperon-Interaktionen mit homogenem Bead-based Assay

Article DOI: 10.3791/62762-v • 06:51 min • July 21st, 2021
July 21st, 2021 •
1, 1, 1,2, 1,3, 1
1Department of Neuroscience Care and Society, Division of Neurogeriatrics, Karolinska Institutet, 2Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology (BHU), 3Theme Inflammation and Aging, Karolinska University Hospital

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Dieses Protokoll stellt eine Technik zur Untersuchung von Protein-Protein-Interaktionen unter Verwendung von Glutathion-verknüpften Spenderperlen mit GST-verschmolzenen TPR-Motiv-Co-Chaperonen und Akzeptorperlen in Verbindung mit einem Hsp90-abgeleiteten Peptid vor. Wir haben diese Technik verwendet, um kleine Moleküle zu screenen, um Hsp90-FKBP51- oder Hsp90-FKBP52-Interaktionen zu stören, und potente und selektive Hsp90-FKBP51-Interaktionshemmer identifiziert.

Tags

Biochemie Ausgabe 173 AlphaScreen Protein-Protein-Interaktionen molekulares Chaperon Hsp90 FKBP51 FKBP52 TPR-Proteine Wirkstoff-Screening
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