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Journal / Neuroscience / 중시경에서 현미경 비늘에 이르기까지 신경 세포 이미징을위한 조직 제거…
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Neuroscience

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JoVE Journal Neuroscience
중시경에서 현미경 비늘에 이르기까지 신경 세포 이미징을위한 조직 제거 방법
Collection of Frozen Rodent Brain Regions for Downstream Analyses
Journal (Neuroscience)
Collection of Frozen Rodent Brain Regions for Downstream Analyses
Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
Journal (Neuroscience)
Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
 
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중시경에서 현미경 비늘에 이르기까지 신경 세포 이미징을위한 조직 제거 방법

Article DOI: 10.3791/63941-v • 07:20 min • May 10th, 2022
May 10th, 2022 •
1,2, 1,2,3, 1,2,4,5, 2,3, 6, 1,2,7
1Department of Neuroanatomy, Juntendo University Graduate School of Medicine, 2Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, 3Advanced Research Institute for Health Sciences, Juntendo University, 4Department of Neuroscience, Graduate School of Medicine, Kyoto University, 5Japan Society for the Promotion of Science, 6Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, 7Department of Multi-Scale Brain Structure Imaging, Juntendo University Graduate School of Medicine

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이 프로토콜은 조직 제거 방법인 ScaleSF를 사용하여 뇌 절편에서 신경 세포 영상의 상세한 방법을 제공한다. 이 프로토콜에는 뇌 조직 준비, 조직 정화, 클리어 슬라이스 처리 및 공초점 레이저 스캐닝 현미경 이미징이 포함됩니다.

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신경과학 문제 183
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